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Part:BBa_I746204:Design

Designed by: Narin Hengrung   Group: iGEM07_Cambridge   (2007-08-31)


FepA L8 Mutant with Elowitz RBS and Standard Terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 21
    Illegal suffix found in sequence at 2240
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 21
    Illegal SpeI site found at 2241
    Illegal PstI site found at 2255
    Illegal NotI site found at 27
    Illegal NotI site found at 2248
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 21
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 21
    Illegal suffix found in sequence at 2241
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 21
    Illegal XbaI site found at 36
    Illegal SpeI site found at 2241
    Illegal PstI site found at 2255
    Illegal AgeI site found at 278
    Illegal AgeI site found at 644
    Illegal AgeI site found at 1271
    Illegal AgeI site found at 1780
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

See BBa_I746200.


Source

L8 FepA was kindly donated to us by Professor P.E. Klebba (see BBa_I746200).

References